Polymorphic microsatellite loci for a neotropical leaf-litter frog (Craugastor bransfordii) characterized through Illumina sequencing
2014
Nowakowski, A Justin | Willoughby, Janna R. | DeWoody, J Andrew | Donnelly, Maureen A.
There are relatively few molecular resources for amphibians in the tropics where widespread habitat loss, disease, and contamination threaten the world’s most diverse assemblages. We used Illumina sequencing to develop microsatellite primers for a dominant amphibian species in Costa Rica that has recently experienced population declines. We characterized 12 polymorphic loci and have provided sequences for over 200 additional primers. These novel molecular resources will be useful for future studies of population genetic structure and may help explain recent declines in one of many species that are decreasing in Central America.
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