Biotransformation of resibufogenin by Actinomucor elegans
2014
Mou, Li-Yan | Xin, Xiu-Lan | Ma, Yukun | Dong, Pei-Pei | Lan, Rong | Su, Dong-Hai | Huang, Jian | Wang, Jin-Hui | Zhan, Li-Bin
Resibufogenin (RB), a major bioactive bufadienolide, has the potential anticancer activity. In the present work, biotransformation of RB by Actinomucor elegans AS 3.2778 yielded five products, namely 3-oxo-resibufogenin (1), 3- epi -resibufogenin (2), 3- epi -12-oxo-hydroxylresibufogenin (3), 3α-acetoxy-15α-hydroxylbufalin (4), and 3- epi -12α-hydroxylresibufogenin (5), respectively. Among them, metabolites 3 and 4 are previously unreported. The chemical structures of metabolites 1 – 5 were fully elucidated on the basis of 2D NMR and HR-MS. The highly stereo - and regio -specific isomerization, hydroxylation, and esterification reactions were observed in the biotransformation process of RB by A. elegans . Their cytotoxicities against A549 and H1299 cells were evaluated.
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