Callus culture from hypocotyls of Kosteletzkya virginica (L.) seedlings: its growth, salt tolerance and response to abscisic acid
1995
Hasson, E. | Poljakoff-Mayber, A.
It was shown that callus established from Kosteletzkya virginica (L.) Presl. (Malvaceae) can grow in salinities higher than 200 mM NaCl if previously accomodated stepwise. Callus lines developed from seedlings of different harvests or of the same harvest at different times, all showed the same pattern of growth and sensitivity to salinity. The absorption of Na+ into the callus increased with increasing external NaCl concentration. In the callus. Na+ was apparently distributed outside and inside a cellular membrane (possibly the plasmalemma). This membrane was, apparently, capable of regulating the Na+ concentration in the protoplast. Outside this membrane Na+ accumulated to concentrations higher than in the external growth medium. Exogenously supplied proline or glycine-betaine did not affect the growth of the callus. Externally applied ABA stimulated growth under saline conditions and increased the accumulation of proline. Growth and proline content were positively correlated in callus exposed to salinity, but in the presence of ABA they were negatively correlated. ABA was involved in both growth and proline accumulation, but there was no clear relationship between these two effects. Both ABA and proline, if added to the growth medium, improved the appearance of the callus.
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