Eggshell matrix proteins as defense mechanism of avian eggs
2003
Mine, Y. | Oberle, C. | Kassaify, Z.
This study focused on the role of eggshell matrix proteins as a function of potential natural antimicrobial defenses of avian eggs. The electrophoretic profile of SDS-PAGE showed that the soluble eggshell matrix proteins had three major bands of 15000, 36000, and 66000 and several minor bands comprising 17000, 25000, 30000, and 75000, while insoluble matrix proteins were consisting of various bands comprising at least 16 distinct migration bands between 10000 and 200000. Three bacteria species, Pseudomonas aeruginosa, Bacillus cereus, and Staphylococcus aureus, were found to be inhibited in the presence of soluble eggshell matrix proteins (100 microgram/mL). On the other hand, Escherichia coli and Salmonella enteritidis were weakly inhibited at only an early stage of incubation time (up to 4 h). Scanning electron microscopy revealed that eggshell matrix proteins might interact and disrupt the membrane integrity of bacteria. The present study clearly indicated that avian eggshell matrix proteins possess a potential of novel antimicrobial defensin mechanism.
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