The effect of aspartic acid on the freeze-drying survival rate of Lactobacillus plantarum LIP-1 and its inherent mechanism
2022
Chen, Zichao | E, Jingjing | Ma, Rongze | Zhang, Jingya | Yao, Caiqing | Wang, Ruixue | Zhang, Qiaoling | Yang, Ying | Li, Jing | Wang, Junguo
Amino acids are often used as growth factors to promote the growth of strains, but we found that adding aspartic acid to the medium could significantly improve the freeze-drying survival rate of some Lactobacillus plantarum (p < 0.05). Our research aimed at Lactobacillus plantarum LIP-1, after exploring its internal mechanism, it is proved that the addition of aspartic acid could significantly reduce the strain's cell wall, cell membrane and DNA damage during the freeze-drying process. Further research concluded some critical points after adding aspartic acid into a medium as below: reduce cell wall damage by increasing the peptidoglycan content, protect the integrity of the cell membrane by increasing the content of long-chain fatty acids, unsaturated fatty acids and cyclopropane fatty acids in cell membranes, and reduce DNA damage by increasing the intracellular pH. Further studies are needed on improving the freeze-drying survival of strains by altering the composition of culture media.
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