Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles
1997
Palmer, K.J. | Tichelaar, W. | Myers, N. | Burns, N.R. | Butcher, S.J. | Kingsman, A.J. | Fuller, S.D. | Saibil, H.R.
The virus-like particles (VLPs) produced by the yeast retrotransposon Ty1 are functionally related to retroviral cores. These particles are unusual in that they have variable radii. A paired mass-radius analysis of VLPs by scanning transmission electron microscopy showed that many of these particles form an icosahedral T-number series. Three-dimensional reconstruction to 38-angstrom resolution from cryo-electron micrographs of T=3 and T=4 shells revealed that the single structural protein encoded by the TYA gene assembles into spiky shells from trimeric units.
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