A comparative study on batch and fed-batch cultures of oleaginous yeast Cryptococcus sp. in glucose-based media and corncob hydrolysate for microbial oil production
2013
Chang, Yi-Huang | Chang, Ku-Shang | Hsu, Chuan-Liang | Chuang, Lu-Te | Chen, Chien-Yu | Huang, Fu-Yu | Jang, Hung-Der
The production of microbial lipids by a batch or fed-batch culture of oleaginous yeast was compared using glucose and corncob hydrolysate as carbon sources. Batch cultures using 4%(w/w) glucose as the carbon source reached a dry biomass, lipid yields and cellular lipid content of 9.4g/l, 6.0g/l and 63.5%(w/w), respectively. However, batch cultures demonstrated that there was an inhibitory effect on the biomass and lipid yields when the glucose concentration increased up to 6%(w/w). It was found that the fed-batch culture diminished the substrate’s inhibition of microbial lipid-accumulation. Fed-batch cultures in the corncob hydrolysate-based media containing an initial 4% reducing sugar and an addition 2% glucose for 4d of cultivation resulted in dry biomass and cellular lipid content of 10.8g/l and 61.3%(w/w), respectively. The overall lipid productivity increased to 1.08g/l/d for the fed-batch culture with an initial 4% glucose in corncob hydrolysate and an addition of corncob hydrolysate containing 2% glucose. High concentrations of reducing sugar in the corncob hydrolysate can be efficiently and rapidly converted to Cryptococcus lipid in the fed-batch culture system. Therefore, microbial lipids from Cryptococcus sp. are prospects for a potential alternative oil resource for biodiesel production.
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