Detection of Bacillus cereus and Pseudomonas fluorescens in dual-species biofilm via real-time PCR and eradication using grapefruit seed extract
2022
Kim, Unji | Kim, Jin Hee | Yi, So-yŏng | Oh, Se-Wook
Foodborne outbreaks are closely associated with the formation of biofilms. However, due to their complexity of the formation and structure, studies of dual-species biofilms are still required. The aim of this study was to analyse the dual-species biofilm of Bacillus cereus and Pseudomonas fluorescens by investigating the interactions between the two species. The results showed that the dual-species biofilm had a higher amount of protein than the mono-species biofilms of the two bacterial species. In addition, in mono-species and dual-species biofilms, the number of B. cereus and P. fluorescens was shown to increase and decrease, respectively, over time. With respect to antimicrobial resistance, the dual-species biofilm required a higher amount of grapefruit seed extract (GSE) than the mono-species biofilms, indicating that the resistance of dual-species biofilm was higher. As only a limited number of studies have investigated the interactions between B. cereus and P. fluorescens using real-time PCR and adopting GSE as an antimicrobial agent, this study may assist the food industries in controlling dual-species biofilms that contain these two bacterial species.
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