Superoxide dismutase detection Anabaena azollae Strasb. isolated from Azolla filiculoides Lam
1991
Canini, A. | Galiazzo, F. | Rotilio, G. | Grilli Caiola, M.
Percoll and sucrose gradients were used to isolate respectively heterocysts and vegetative cells of Anabaena azollae symbiont of Azolla filiculoides. Both heterocysts and vegetative cells devoid of contaminating bacteria and Azolla debris, were used to assay the presence of the enzyme superoxide dismutase (SOD) by activity-stained polyacrylamide gel electrophoresis. Types of SOD were determined by different sensitivity to 2 millimole cyanide and 2 millimole hydrogen peroxide. Heterocysts contained only a faint band of both hybrid iron-manganese (Hy) and iron (Fe) superoxide dismutase. Vegetative cells possess Mn-SOD, HY-SOD and Fe-SOD. HY-SOD is detected for the first time in cyanobacteria. The higher activity was present as Fe-SOD in vegetative cells while total heterocysts SOD activity was only 15% with respect to vegetative cells.
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