Modified DPPH and ABTS Assays to Assess the Antioxidant Profile of Untreated Oils
2015
Christodouleas, Dionysios C. | Fotakis, Charalambos | Nikokavoura, Aspasia | Papadopoulos, Kyriakos | Calokerinos, Antony C.
This research dwells on two widely used spectrophotometric methods, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, which assess the free radical scavenging activity (RSA) of natural samples and standard compounds. In particular, these assays were modified in order to simplify the evaluation of RSA of untreated edible oils, as well as to assess the antioxidant profile of oils’ hydrophilic and lipophilic extracts with the same analytical procedure. A thorough study highlighted the effect of solvents on the DPPH and ABTS methods and resulted in selecting 2-propanol and an ethanol/1-butanol solvent mixture as the reaction solvent for the DPPH method and the ABTS method, respectively. The developed methods were used to evaluate the RSA of 12 antioxidant compounds and 8 edible oils. Then, the contribution of lipophilic and hydrophilic extracts to the total RSA of oils was estimated. The obtained results demonstrate the applicability of the method to routine edible oil analysis.
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