Defining the requirements for bovine embryo culture
1996
Thompson, J.G.
There are several culture systems which support the development of bovine embryos in vitro. By far the most popular method is co-culture with somatic cells, but increasingly defined mono-culture systems are also a practical alternative. To develop a deemed system requires knowledge of: the biochemical requirements for embryo development; the composition of the in vivo environment. Neither of these are constant during embryo development, yet most laboratories continue to grow embryos under relatively static conditions. A new approach is to perfuse embryos with a medium in which gradient changes in both physical and chemical conditions can be introduced over time. Perfusion culture of embryos offers a number of advantages over static conditions and it is proposed that such systems will become increasingly popular in the future.
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