Real-time PCR for differential determination of the tomato wilt fungus, Fusarium oxysporum f. sp. lycopersici, and its races
2010
Inami, Keigo | Yoshioka, Chizu | Hirano, Yasushi | Kawabe, Masato | Tsushima, Seiya | Teraoka, Tohru | Arie, Tsutomu
Five primer/probe sets to identify the tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici (FOL), and its three races selectively were designed based on the rDNA-intergenic spacer and avirulence genes. Real-time PCR using genomic DNA from mycelia and soil DNA with the primer/probe sets allowed the successful identification of FOL and its races.
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