Évaluation de l’activité antifongique de différents extraits de la cannelle de Chine (Cinnamomum cassia) | Evaluation of the antifungal activity of different extracts of Chinese cinnamon (Cinnamomum cassia)
2012
Merghache, D. | Boucherit-Atmani, Z. | Boucherit, K.
The incidence of invasive fungal infections due to Candida albicans has dramatically increased since 25 years. The amphotericin B remains the best treatment despite its severe toxicity. Our work is inscribed in the frame of finding of new natural antifungals agents from a condiment widely used in our diet: the Chinese cinnamon (Cinnamomum cassia). This study is focused on the qualitative determination of different families of secondary metabolites from the bark of Cinnamon. It is also focused on assessing the antifungal activity of some extracts of Cinnamon. The plant material was extracted by exhaustion using increased polarity solvents (chloroform, ethyl acetate, methanol and water). We made five exhaustions for each solvent, each one was tested separately. The phytochemical study revealed the presence of terpenes, alkaloids and polyphenols mainly represented by flavonoids. Evaluation of antimicrobial activity of the various extracts was carried out against references yeasts strains Candida albicans ATCC 10231 and Candida albicans 444IP. Results showed that in the exception of aqueous extracts, all other extracts have an interesting activity, with inhibition zone diameters between 19 and 60 mm for the chloroform extract. Similar results were obtained for the other organic extracts. Indeed, extracts obtained from low or medium polarity solvents are the most active. In addition, the MIC and MFC of the first fraction of the chloroform extract were respectively 0.10 and 0.20 μg/ml against Candida albicans ATCC 10231. They remain lower than those of amphotericin B against the same strain (MIC = 0.2 μg/ml and MFC = 0.4 μg/ml).
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