Antigenic and molecular characterization of Yersinia ruckeri proposal for a new intraspecies classification
1993
Romalde, J.L. | Magariños, B. | Barja, J.L. | Toranzo, A.E.
The serological and molecular properties of a group of 17 Yersinia ruckeri strains isolated from cultured rainbow trout in Spain were compared with a total of 36 collection strains isolated from fish and other geographic areas. The serological relationship among isolates, as well as the study of their antigenic determinants (LPS and membrane proteins) allow to propose a new serotyping scheme with four O-serotypes (from O1 to O4). Moreover, serotypes O1 and O2 were divided in two and three groups respectively based mainly on minor differences observed in LPS and membrane protein profiles. The patterns obtained in the analysis of the LPS and proteins present in the extracellular products also support the serological scheme proposed. The strains showed a great genetic homogeneity, displaying similar DNA fingerprint patterns regardless of their serotype. In contrast, only the serotype O1 isolates harbour a plasmid of about 50 MDa, while the strains of the other serotypes presented no extrachromosomical DNA or only small plasmids (less than 10 MDa).
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