Detection of Cross‐Reactive Antigens between Phytophthora infestans (Mont.) de Bary and Solanum Species by Indirect Enzyme‐Linked Immunosorbent Assay
1985
Alba, A.P.C. | DeVay, J.E.
Cross‐reactive antigens were detected in crude preparations and in purified preparations from mycelia of Phytophthora infestans Race 4. and Race 1.2.3.4.7. with antisera for potatoes cv. King Edward and cv. Pentland Dell by using an indirect ELISA technique. Results suggest that the fungal mycelia do not easily release cross‐reactive antigens into synthetic media where they grow; that most of P. infestans cross‐reactive antigens are thermolabile and that they can be concentrated by precipitation in the presence of 40 % saturated ammonium sulphate (SAS). The results also reveal an antigenic disparity when 40 % SAS from P. infestans Race 4. mycelial preparation was assayed with antisera for King Edward and Pentland Dell. The occurrence of cross‐reactive antigens in P. infestans mycelium and their involvement in such interaction are discussed.
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