Differential expression of NBS-LRR-encoding genes in the root transcriptomes of two Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis
2017
Kochetov, AlexV. | Glagoleva, AnastasiyaY. | Strygina, KseniyaV. | Khlestkina, ElenaK. | Gerasimova, SophiaV. | Ibragimova, SalmazM. | Shatskaya, NataljaV. | Vasilyev, GennadyV. | Afonnikov, DmitryA. | Shmakov, NikolayA. | Antonova, OlgaY. | Gavrilenko, TatyanaA. | Alpatyeva, NataliaV. | Khiutti, Alexander | Afanasenko, OlgaS.
BACKGROUND: The characterization of major resistance genes (R genes) in the potato remains an important task for molecular breeding. However, R genes are rapidly evolving and frequently occur in genomes as clusters with complex structures, and their precise mapping and identification are complicated and time consuming. RESULTS: Comparative analysis of root transcriptomes of Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis revealed a number of differentially expressed genes. However, compiling a list of candidate R genes for further segregation analysis was hampered by their scarce annotation. Nevertheless, combination of transcriptomic analysis with data on predicted potato NBS-LRR-encoding genes considerably improved the quality of the results and provided a reasonable number of candidate genes that provide S. phureja with strong resistance to the potato golden cyst nematode. CONCLUSION: Combination of comparative analyses of tissue-specific transcriptomes in resistant and susceptible genotypes may be used as an approach for the rapid identification of candidate potato R genes for co-segregation analysis and may be used in parallel with more sophisticated studies based on genome resequencing.
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