Effects of four egg desticking procedures on hatching rate and further survival and growth of larvae in the tench (Tinca tinca L.)
2006
Carral, Jose M. | Celada, Jesús D. | Sáez-Royuela, Maria | Rodriguez, Ruth | Aguilera, Amelia | Melendre, Pedro
Four desticking procedures for tench eggs (A: tannic acid solution (1 g L[superscript [-]1]) for 15 s; B: alcalase enzyme solution (8 mL L[superscript [-]1]) for 60 s; C: alcalase enzyme solution (15 mL L[superscript [-]1]) for 120 s; D: Woynarovich and Woynarovich (1980) solution for 58 min followed by tannic acid solution (1 g L[superscript [-]1]) for 15 s) were tested to obtain data about influence on embryo survival to hatching stage and further survival and growth of the larvae. In the tannic acid and Woynarovich and Woynarovich (1980) treatment (A and D) few eggs stuck together and some were adhered to the incubator walls, whereas in the alcalase treatments (B and C) eggs neither stuck together nor adhered to the incubator walls. Percentages of hatched larvae did not show significant differences (mean values ranged between 47.4% in treatment A to 37.0% in treatment C). Larvae deformities observed were <0.5% in all cases. There were no significant differences among survival and growth rates of the larvae from different egg desticking origin, reaching, after 30 days, mean survival values around 90% and total length and weight of 12.5 mm and 19 mg respectively.
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