Sporicidal Action of Hypochlorite on Conidia of Aspergillus parasiticus
1980
Ver Kuilen, Stephen D. | Marth, E. B.
The sporicidal action of sodium hypochlorite was determined using conidiospores of Aspergillus parasiticus NRRL 2999 and NRRL 3315. Conidia were harvested after the mold grew 10 days on Moyer's or mycological agar. The conidia were suspended in phosphate buffer solutions at pH 5, 6, 7 and 8, and heated to 30 or 40 C. Initial trials were made with 3 ppm of free residual chlorine. Other experiments were done with 50 ppm of chlorine added to solutions containing 0.10 or 0.25% tryptone or 1% sucrose or glucose. Surviving conidia were enumerated with mycological agar. When tryptone was absent, chlorine at 3 ppm was more lethal to conidia than was 50 ppm when tryptone was present. Generally, survival of conidia was substantially greater at pH 8 and 30 C than at pH 5, 6 and 7 where survival was similar. When tryptone was present, substantial numbers of conidia survived at pH 7 and 8. Chlorine was markedly more lethal at 40 than 30 C at all pH values. No conidia survived in the presence of 1% sucrose and 50 ppm of chlorine.
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