Nanopore Sequencing as a Rapid Tool for the Identification of Cucumber Mosaic Virus Infecting Spearmint (Mentha spicata)
2023
Lee, H.J. | Cho, I.S. | Jeong, R.D.
Spearmint (Mentha spicata L.) is one of the most popular mint species in South Korea due to its range of beneficial effects. We collected spearmint plants that exhibited virus-infection-like mosaic and chlorosis foliar symptoms and identified the causal viral pathogen using an Oxford Nanopore Technologies (ONT)-based MinION platform, which is a compact and portable rapid sequencing device. The nearly-complete genomic sequence of the cucumber mosaic virus (CMV) infecting spearmint was determined and consisted of RNA 1 (3120 nt) with ORF1a encoding 991 amino acids, RNA 2 (2968 nt) with ORF2a encoding 857 and ORF2b encoding 111 aa, and RNA 3 (2128 nt) with ORF3a encoding 280 and ORF3b encoding 219 aa. Phylogenetic analysis based on complete coat protein sequences revealed that CMV-CNU-spearmint was a member of the subgroup IA. This is the first report of a natural CMV infection of spearmint in South Korea. The rapid and reliable sequencing tools provided by the ONT can facilitate the surveillance and identification of plant viruses in the field.
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