DNA barcodes in Egyptian olive cultivars (Olea europaea L.) using the rbcL and matK coding sequences
2023
Said, E.M. | Hassan, M.E.
Olives (Olea europaea L.) are the most well-known trees of the Mediterranean basin. The genetic heritage of olive trees is very rich and characterized by an abundance of varieties, eventually leading to significant genotype mixing. Therefore, the classification and analysis of the genetic diversity of olive cultivars are challenges of high priority. It helps make more efficient use of the olive resources distributed globally and benefits the genetic improvement and breeding of olive varieties. DNA barcoding is a method for evaluating and classifying species that uses short, variable, and uniform DNA regions. This study used DNA sequencing data for the first time for DNA barcoding of Egyptian olive cultivars and assessed the genetic relationship between four local cultivars grown in Egypt and their relation to sequences within the database. PCR amplification and sequence characterization were used to assess the effectiveness of the two barcoding sites, rbcL and matK. Based on multiple alignments of all the sequences obtained from the analyzed regions, matK had the longest sequence (816 bp) and rbcL the shortest (411 bp) of the two barcoding loci. The matK gene region was the least varied, with a variability level of 77%, while the rbcL gene region had the highest amount of variability (98.7%). Because of the presence of unique variable sites, both barcoding loci rbcL and matK efficiencies discriminate the selective Egyptian olive cultivars. The DNA sequences from the individual and combined barcode loci were used in a maximum likelihood tree-based phylogenetic analysis to successfully distinguish the selected olive cultivars.
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