Inhibition of Corynebacterium pseudotuberculosis biofilm by DNA synthesis and protein synthesis inhibitors
2022
Faez Firdaus Abdullah Jesse | Mohamad Fakhri Yaacob | Mohd Fakharul Zaman Raja Yahya | Syaida Anati Abd Rashid | Mohd Shafiq Aazmi
Corynebacterium pseudotuberculosis represents the causative factor of caseous lymphadenitis, a ruminant disease that contributes to major economic loss in most sheep farming countries. The present study was performed to determine the efficacy of DNA synthesis (nalidixic acid) and protein synthesis inhibitors (streptomycin and tetracycline) on C. pseudotuberculosis biofilm. Biofilm of C. pseudotuberculosis clinical isolate was developed in the flat-bottom microplate. Field-emission scanning electron microscope and resazurin assay were used to investigate the structure and antimicrobial susceptibility of C. pseudotuberculosis biofilm, respectively. All test inhibitors were evaluated in the range between 3.125 µg/ml and 100 µg/ml. Results demonstrated that C. pseudotuberculosis biofilm formed a three dimensional and heterogeneous structure on the surface. The highest biofilm percentage inhibition shown by nalidixic acid, streptomycin and tetracycline were 71.57%, 87.44% and 74.73%, respectively. Significant (p<0.05) correlation of antibiofilm susceptibility between these inhibitors and other antimicrobials were also demonstrated herein. The findings of the present study suggest the potential use of streptomycin for the control of caseous lymphadenitis.
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