Structural studies of the cell wall polysaccharides from three strains of Lactobacillus helveticus with different autolytic properties: DPC4571, BROI, and LH1
2013
Vinogradov, Evgeny | Valence, Florence | Maes, Emmanuel | Jevaba, Iva | Chuat, Victoria | Lortal, Sylvie | Grard, Thierry | Guerardel, Yann | Sadovskaya, Irina | National Research Council of Canada (NRC) | Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF) ; Université de Lille-Centre National de la Recherche Scientifique (CNRS) | Science et Technologie du Lait et de l'Oeuf (STLO) ; Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Institut National de la Recherche Agronomique (INRA) | ICT, Department of Dairy and Fat Technology ; Faculty of Food and Biochemical Technology | Agroalimentaire et Produits Aquatiques (APA) ; Université du Littoral Côte d'Opale (ULCO) | INRA and Ministry of Education, Youth and Sports of the Czech Republic (Grant No MSMT6046137305) and Région Nord-Pas de Calais grant ARCIR 54R01. The 800 and 900 MHz spectrometers were funded by Région Nord-Pas de Calais, European Union (FEDER), Ministère Français de la Recherche, Université Lille 1 and CNRS. Financial support from the TGE RMN THC FR-3050
Lactobacillus helveticus is traditionally used in dairy industry as a starter or an adjunct culture for manufacture of cheese and some types of fermented milk. Its autolysis releases intracellular enzymes which is a prerequisite for optimum cheese maturation, and is known to be strain dependent. Autolysis is caused by an enzymatic hydrolysis of the cell wall peptidoglycan (PG) by endogenous peptidoglycan hydrolases (PGHs) or autolysins. Origins of differences in autolytic properties of different strains are not fully elucidated. Regulation of autolysis possibly depends on the structure of the cell wall components other than PG, particularly polysaccharides. In the present work, we screened six L. helveticus strains with different autolytic properties: DPC4571, BROI and LH1. We established, for the first time, that cell walls (CWs) of these strains contained polysaccharides, different from their CW teichoic acids. Cell wall polysaccharides of three strains were purified, and their chemical structures were established by 2D NMR spectroscopy and methylation analysis. The structures of their repeating units are presented.
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