SmCSP4 from aphid saliva stimulates salicylic acid‐mediated defence responses in wheat by interacting with transcription factor TaWKRY76
2023
Zhang, Yong | Fu, Yu | Liu, Xiaobei | Francis, Frédéric | Fan, Jia | Liu, Huan | Wang, Qian | Sun, Yu | Zhang, Yumeng | Chen, Julian | Institute of Plant Protection [CAAS] (IPP) ; Chinese Academy of Agricultural Sciences (CAAS) | Plant Health Institute of Montpellier (UMR PHIM) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) | Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] ([FUSAGx]) ; Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège) | China Agricultural University (CAU) | Qingdao Agricultural University | National Key R & D Program (2023YFD1700304) | the National Natural Science Foundation of China (Nos. 31871979, 31901881) | Agricultural Science and Technology Innovation Program (CAAS-ZDRW202108) | China's Donation to the CABI Development Fund (IVM10051).
International audience
Mostrar más [+] Menos [-]Inglés. Aphid salivary proteins are critical in modulating plant defence responses. Grain aphid Sitobion miscanthi is an important wheat pest worldwide. However, the molecular basis for the regulation of the plant resistance to cereal aphids remains largely unknown. Here, we show that SmCSP4, a chemosensory protein from S. miscanthi saliva, is secreted into wheat plants during aphid feeding. Delivery of SmCSP4 into wheat leaves activates salicylic acid (SA)-mediated plant defence responses and subsequently reduces aphid performance by deterring aphid feeding behaviour. In contrast, silencing SmCSP4 gene via nanocarrier-mediated RNAi significantly decreases the ability of aphids to activate SA defence pathway. Protein-protein interaction assays showed that SmCSP4 directly interacts with wheat transcriptional factor TaWRKY76 in plant nucleus. Furthermore, TaWRKY76 directly binds to the promoter of SA degradation gene Downy Mildew Resistant 6 (DMR6) and regulates its gene expression as transcriptional activator. SmCSP4 secreted by aphids reduces the transcriptional activation activity of TaWRKY76 on DMR6 gene expression, which is proposed to result in increases of SA accumulation and enhanced plant immunity. This study demonstrated that SmCSP4 acts as salivary elicitor that is involved in activating SA signalling defence pathway of wheat by interacting with TaWRKY76, which provide novel insights into aphid-cereal crops interactions and the molecular mechanism on induced plant immunity.
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