Freezing stallion semen in INRA96®-based extender improves fertility rates in comparison with INRA82 | La congélation de semence d'étalon dans un milieu à base d'INRA96® améliore le taux de fertilité en comparaison avec le milieu INRA82
2008
Pillet, Elodie | Batellier, Florence | Duchamp, Guy | Furstoss, Vincent, V. | Le Vern, Yves | Kerboeuf, Dominique | Vidament, Marianne | Magistrini, Michèle | Physiologie de la reproduction et des comportements [Nouzilly] (PRC) ; Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) | IMV Technologies | Unité Pluri-espèces d'Expérimentation Animale en Physiologie de la Reproduction et des Comportements (TOURS UPEA PRC) ; Institut National de la Recherche Agronomique (INRA) | Insémination Caprine et Porcine (ICP) ; Institut National de la Recherche Agronomique (INRA) | Infectiologie Animale et Santé Publique (UR IASP) ; Institut National de la Recherche Agronomique (INRA)
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Mostrar más [+] Menos [-]Inglés. The chemical composition of freezing extenders plays a major role in sperm cell survival during cryopreservation. In this study we compared two extenders for freezing stallion semen: INRA82 extender (as a control) versus INRA96 (R) extender, both supplemented with egg yolk and glycerol. INRA82 contains milk, whereas INRA96 (R) is a chemically- defined extender developed for fresh semen storage at 4 degrees C or 15 degrees C. Semen from 3 stallions (7 ejaculates per stallion) was frozen in both extenders. In vitro analyses of post-thaw motility of sperm cells (computer-assisted analysis) and of membrane integrity (flow cytometry analysis) were performed. Then a fertility trial was conducted. Inseminations were conducted in a total of 84 mare cycles. INRA96 (R) extender supplemented with egg yolk and glycerol significantly improved per-cycle pregnancy rates compared with INRA82 (71% versus 40%, p < 0.01). In agreement with these fertility results, membrane integrity was better preserved in INRA96 (R) than in INRA82. In contrast, motility parameters were significantly higher in INRA82 than in INRA96 (R). Further research is needed to understand how INRA96 (R) components protect sperm cells during the cryopreservation process and highly increase their fertility potential compared with INRA82 components.
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