Cloning and sequencing of porcine LH-hCG receptor cDNA: variants lacking transmembrane domain
1989
Loosfelt, Hugues | Misrahi, Micheline | Atger, Michel | Salesse, Roland | Vu Hai-Luu Thi, Mai Tu | Jolivet, André | Guiochon-Mantel, Anne | Sar, Sokhavuth | Jallal, Bahija | Garnier, Jean | Milgrom, Edwin | Hormones, gènes et reproduction ; Institut National de la Santé et de la Recherche Médicale (INSERM) | Hôpital Bicêtre [AP-HP, Le Kremlin-Bicêtre] ; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP) | Unité de biologie cellulaire et moléculaire ; Institut National de la Recherche Agronomique (INRA) | Pathogénèse et traitement de l'hépatite fulminante et du cancer du foie ; Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM)
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Mostrar más [+] Menos [-]Inglés. Complementary DNA clones, encoding the LH-hCG (luteinizing hormone-human choriogonadotropic hormone) receptor were isolated by screening a λgt11 library with monoclonal antibodies. The primary structure of the protein was deduced from the DNA sequence analysis; the protein contains 696 amino acids with a putative signal peptide of 27 amino acids. Hydropathy analysis suggests the existence of seven transmembrane domains that show homology with the corresponding regions of other G protein-coupled receptors. Three other types of clones corresponding to shorter proteins were observed, in which the putative transmembrane domain was absent. These probably arose through alternative splicing. RNA blot analysis showed similar patterns in testis and ovary with a major RNA of 4700 nucleotides and several minor species. The messenger RNA was expressed in COS-7 cells, yielding a protein that bound hCG with the same affinity as the testicular receptor.
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