Synchrotron FTIR and Raman spectroscopy provide unique spectral fingerprints for <em>Arabidopsis</em> floral stem vascular tissues
2019
Dinant, Sylvie | Wolff, Nelly | de Marco, Federica | Vilaine, Françoise | Gissot, Lionel | Aubry, Emilia | Sandt, Christophe | Bellini, Catherine | Le Hir, Rozenn | Institut Jean-Pierre Bourgin (IJPB) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Université Paris Saclay (COmUE) | Centre National de la Recherche Scientifique (CNRS) | Synchrotron SOLEIL (SSOLEIL) ; Centre National de la Recherche Scientifique (CNRS) | Umea Plant Science Centre, Department of Plant Physiology ; Umeå University = Umeå Universitet | SOLEIL, French national synchrotron facility [20150210] ; French State grant [LabEx Saclay Plant Sciences-SPS, ANR-10-LABX512 0040-SPS], managed by the French National Research Agency under an "Investments for the Future" program [ANR-11-IDEX-0003-02] ; Agropolis Foundation [ID-1401-005] through the "Investissements d'Avenir" program [Labex Agro: ANR516 10-LABX-0001-01] | ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017) | ANR-10-LABX-0001,AGRO,Agricultural Sciences for sustainable Development(2010) | ANR-11-IDEX-0003,IPS,Idex Paris-Saclay(2011) | ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010)
Preprint publié dans BioRxiv : https://www.biorxiv.org/content/early/2018/06/11/343343 https://prodinra.inra.fr/record/432127
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Mostrar más [+] Menos [-]Inglés. Cell walls are highly complex structures that are modified during plant growth and development. For example, the development of phloem and xylem vascular cells, which participate in the transport of sugars and water as well as providing support, can be influenced by cell-specific wall composition. Here, we used synchrotron radiation-based Fourier-transform infrared (SR-FTIR) and Raman spectroscopy to analyse the cell wall composition of floral stem vascular tissues of wild-type Arabidopsis and the double-mutant sweet11-1 sweet12-1, which has impaired sugar transport. The SR-FTIR spectra showed that in addition to modified xylem cell wall composition, phloem cell walls in the double-mutant line were characterized by modified hemicellulose composition. Combining Raman spectroscopy with a classification and regression tree (CART) method identified combinations of Raman shifts that could distinguish xylem vessels and fibers. In addition, the disruption of the SWEET11 and SWEET12 genes impacted on xylem wall composition in a cell-specific manner, with changes in hemicelluloses and cellulose observed at the xylem vessel interface. These results suggest that the facilitated transport of sugars by transporters that exist between vascular parenchyma cells and conducting cells is important in ensuring correct phloem and xylem cell wall composition.
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