Massive crossover elevation via combination of <em>HEI10</em> and <em>recq4a recq4b</em> during <em>Arabidopsis</em> meiosis
2018
Serra, Heïdi | Lambing, Christophe | Griffin, Catherine H. | Topp, Stephanie D. | Nageswaran, Divyashree C. | Underwood, Charles J. | Ziolkowski, Piotr A. | Séguéla Arnaud, Mathilde | Fernandes, Joiselle B. | Mercier, Raphaël | Henderson, Ian R. | Department of Plant Sciences ; University of Cambridge [UK] (CAM) | Institut Jean-Pierre Bourgin (IJPB) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Université Paris Saclay (COmUE) | Université Paris-Sud - Paris 11 (UP11) | Biotechnology and Biological Sciences Research Council (BBSRC) [BB/L006847/1] ; BBSRC-Meiogenix IPA [BB/N007557/1] ; European Research Area Network for Coodinating Action in Plant Sciences/BBSRC "DeCOP" [BB/M004937/1] ; European Research Council (SynthHotspot CoG) ; Gatsby Charitable Foundation [GAT2962] ; Royal Society University Research Fellowship ; Bettencourt Schueller Foundation | European Project: 606956,EC:FP7:PEOPLE,FP7-PEOPLE-2013-ITN,COMREC(2013)
International audience
Mostrar más [+] Menos [-]Inglés. During meiosis, homologous chromosomes undergo reciprocal crossovers, which generate genetic diversity and underpin classical crop improvement. Meiotic recombination initiates from DNA double-strand breaks (DSBs), which are processed into single-stranded DNA that can invade a homologous chromosome. The resulting joint molecules can ultimately be resolved as crossovers. In <em>Arabidopsis</em>, competing pathways balance the repair of ∼100-200 meiotic DSBs into ∼10 crossovers per meiosis, with the excess DSBs repaired as noncrossovers. To bias DSB repair toward crossovers, we simultaneously increased dosage of the procrossover E3 ligase gene <em>HEI10</em> and introduced mutations in the anticrossovers helicase genes <em>RECQ4A</em> and <em>RECQ4B</em>. As <em>HEI10</em> and <em>recq4a recq4b</em> increase interfering and noninterfering crossover pathways, respectively, they combine additively to yield a massive meiotic recombination increase. Interestingly, we also show that increased <em>HEI10</em> dosage increases crossover coincidence, which indicates an effect on interference. We also show that patterns of interhomolog polymorphism and heterochromatin drive recombination increases distally towards the sub-telomeres in both <em>HEI10</em> and <em>recq4a recq4b</em> backgrounds, while the centromeres remain crossover suppressed. These results provide a genetic framework for engineering meiotic recombination landscapes in plant genomes.
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