Fast digestive, leucine-rich, soluble milk proteins improve muscle protein anabolism and mitochondrial function in undernourished old rats
2017
Salles, Jérôme | Chanet, Audrey | Berry, Alexandre | Giraudet, Christophe | Patrac, Véronique | Domingues-Costa Faria, Carla | Rocher, Christophe | Guillet, Christelle | Denis, Philippe | Pouyet, Corinne | Bonhomme, Cécile | Ruyet, Pascale Le | Rolland, Yves | Boirie, Yves | Walrand, Stephane | Unité de Nutrition Humaine (UNH) ; Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]) | Institut de biochimie et génétique cellulaires (IBGC) ; Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS) | Centre National de la Recherche Scientifique (CNRS) | Université de Bordeaux (UB) | Groupe Lactalis | Institut du Vieillissement ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT) | Epidémiologie et analyses en santé publique : risques, maladies chroniques et handicaps (LEASP) ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM) | Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT) | Service Nutrition Clinique [CHU Clermont-Ferrand] ; Pôle RHEUNNIRS [CHU Clermont-Ferrand] ; CHU Gabriel Montpied [Clermont-Ferrand] ; CHU Clermont-Ferrand-CHU Clermont-Ferrand-CHU Gabriel Montpied [Clermont-Ferrand] ; CHU Clermont-Ferrand-CHU Clermont-Ferrand | French National Agency for Research (ANR)
in press
Mostrar más [+] Menos [-]Inglés. SCOPE: One strategy to manage malnutrition in older patients is to increase protein and energy intake. Here we evaluate the influence of protein quality during refeeding on improvement in muscle protein and energy metabolism.METHODS AND RESULTS: Twenty-month-old male rats (n = 40) were fed 50% of their spontaneous intake for 12 weeks to induce malnutrition, then refed ad libitum with a standard diet enriched with casein or soluble milk proteins (22%) for 4 weeks. 13C-valine was infused to measure muscle protein synthesis and expression of MuRF1, and MAFbx was measured to evaluate muscle proteolysis. mTOR pathway activation and mitochondrial function were assessed in muscle. Malnutrition was associated with a decrease in body weight, fat mass and lean mass, particularly muscle mass. Malnutrition decreased muscle mTOR pathway activation and protein FSR associated with increased MuRF1 mRNA levels, and decreased mitochondrial function. The refeeding period partially restored fat mass and lean mass. Unlike the casein diet, the soluble milk protein diet improved muscle protein metabolism and mitochondrial function in old malnourished rats.CONCLUSIONS: These results suggest that providing better-quality proteins during refeeding may improve efficacy of renutrition in malnourished older patients. This article is protected by copyright. All rights reserved.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Institut national de la recherche agronomique