Deciphering the Theobroma cacao self-incompatibility system: from genomics to diagnostic markers for self-compatibility
2017
Lanaud, Claire | Fouet, Olivier | Legavre, Thierry | Lopes, Uilson | Sounigo, Olivier | Eyango, Marie Claire | Mermaz, Benoit | da Silva, Marcos Ramos | Solorzano, Rey Gaston Loor | Argout, Xavier | Gyapay, Gabor | Ebaiarrey, Hermann Ebai | Colonges, Kelly | Sanier, Christine | Rivallan, Ronan | Mastin, Géraldine | Cryer, Nicholas | Boccara, Michel | Verdeil, Jean-Luc | Mousseni, Ives Bruno Efombagn | Gramacho, Karina Peres | Clement, Didier | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Département Systèmes Biologiques (Cirad-BIOS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Centro de Pesquisas do Cacau - CEPLAC (BRAZIL) ; Centro de Pesquisas do Cacau - CEPLAC (BRAZIL) | Bioagresseurs, analyse et maîtrise du risque (UPR Bioagresseurs) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Institut de Recherche Agricole pour le Développement [Yaoundé] (IRAD) | Instituto Nacional de Investigaciones Agropecuarias (INIAP) | Genoscope - Centre national de séquençage [Evry] (GENOSCOPE) ; Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) | Institut de Génomique d'Evry (IG) ; Université Paris-Saclay-Institut de Biologie François JACOB (JACOB) ; Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) | Mondelez International R&D, Global RDQ Research & Nutrition | This project was supported by Agropolis Fondation under the reference ID 1403-46 through the 'Investissements d'avenir' programme (Labex Agro: ANR-10-LABX-0001). We also thank Mondelez International for their financial contribution to this project. | ANR-10-LABX-0001,AGRO,Agricultural Sciences for sustainable Development(2010)
International audience
Mostrar más [+] Menos [-]Inglés. Cocoa self-compatibility is an important yield factor and has been described as being controlled by a late gameto-sporophytic system expressed only at the level of the embryo sac. It results in gametic non-fusion and involves several loci. In this work, we identified two loci, located on chromosomes 1 and 4 (CH1 and CH4), involved in cocoa self-incompatibility by two different processes. Both loci are responsible for gametic selection, but only one (the CH4 locus) is involved in the main fruit drop. The CH1 locus acts prior to the gamete fusion step and independently of the CH4 locus. Using fine-mapping and genome-wide association studies, we focused analyses on restricted regions and identified candidate genes. Some of them showed a differential expression between incompatible and compatible reactions. Immunolocalization experiments provided evidence of CH1 candidate genes expressed in ovule and style tissues. Highly polymorphic simple sequence repeat (SSR) diagnostic markers were designed in the CH4 region that had been identified by fine-mapping. They are characterized by a strong linkage disequilibrium with incompatibility alleles, thus allowing the development of efficient diagnostic markers predicting self-compatibility and fruit setting according to the presence of specific alleles or genotypes. SSR alleles specific to self-compatible Amelonado and Criollo varieties were also identified, thus allowing screening for self-compatible plants in cocoa populations.
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