In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella
2022
Koczerka, Michael | Lantier, Isabelle | Pinard, Anne | Morillon, Marie | Deperne, Justine | Gal-Mor, Ohad | Grépinet, Olivier | Virlogeux-Payant, Isabelle | Infectiologie et Santé Publique (ISP) ; Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Plateforme d'Infectiologie Expérimentale (PFIE) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | The Infectious Diseases Research Laboratory ; Chaim Sheba Medical Center | Tel Aviv University (TAU) | INRAE; Université de Tours | Ohad Gal-Mor | ANR-15-IFEC-0003,Sal host trop,Understanding the Human-Restricted Host Tropism of Typhoidal Salmonella(2015) | European Project: 731014,VetBioNet(2017)
chapitre 19
Mostrar más [+] Menos [-]International audience
Mostrar más [+] Menos [-]Inglés. Applications of bioluminescence for the in vivo study of pathogenic microorganisms are numerous, ranging from the quantification of virulence gene expression to measuring the effect of antimicrobial molecules on the colonization of tissues and organs by the pathogen. Most studies are performed in mice, but recent works demonstrate that this technique is applicable to larger animals like fish, guinea pigs, ferrets, and chickens. Here, we describe the construction and the utilization of a constitutively luminescent strain of Salmonella Typhimurium to monitor in vivo and ex vivo the colonization of mice in the gastroenteritis, typhoid fever, and asymptomatic carriage models of Salmonella infection.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Institut national de la recherche agronomique