Role of mechanical vs. chemical action in the removal of adherent Bacillus spores during CIP procedures
2013
Faille, C. | Bénézech, T. | Blel, W. | Ronse, A. | Ronse, G. | Clarisse, M. | Slomianny, C. | Laboratoire d'Ingénierie des Matériaux de Bretagne (LIMATB) ; Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Brestois du Numérique et des Mathématiques (IBNM) ; Université de Brest (UBO)-Université de Brest (UBO) | Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA) ; Mines Nantes (Mines Nantes)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST) ; Université de Nantes (UN)-Université de Nantes (UN)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire de Génie des Procédés et Technologie Alimentaires (LGPTA) ; Institut National de la Recherche Agronomique (INRA) | Agence Nationale de la Recherche [ANR-07-PNRA-009]
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Mostrar más [+] Menos [-]Inglés. This study was designed to evaluate the respective roles of mechanical and chemical effects on the removal of Bacillus spores during cleaning-in-place. This analysis was performed on 12 strains belonging to the Bacillus cereus group (B. cereus, Bacillus anthracis, Bacillus thuringiensis) or to less related Bacillus species (Bacillus pumilus, Bacillus licheniformis, Bacillus sporothermodurans, Bacillus subtilis). Adherent spores were subjected to rinsing-in-place (mechanical action) and cleaning-in-place (mechanical and chemical actions) procedures, the latter involving NaOH 0.5% at 60°C. Results revealed that mechanical action alone only removed between 53 and 89% of the attached spores at a shear stress of 500 Pa. This resistance to shear was not related to spore surface properties. Conversely, in the presence of NaOH at a shear stress of 4 Pa, spores were readily detached, with between 80 and 99% of the adherent spores detached during CIP and the chemical action greatly depended on the strain. This finding suggests that chemical action plays the major role during CIP, whose efficacy is significantly governed by the spore surface chemistry.
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