RNA Viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles
2020
Gondard, Mathilde | Temmam, Sarah | Devillers, Elodie | Pinarello, Valérie | Bigot, Thomas | Chrétien, Delphine | Aprelon, Rosalie | Vayssier Taussat, Muriel | Albina, Emmanuel | Eloit, Marc | Moutailler, Sara | Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR) ; École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Découverte de Pathogènes - Pathogen Discovery ; Biologie des Infections - Biology of Infection ; Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM) | Département Systèmes Biologiques (Cirad-BIOS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB ; Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) | École nationale vétérinaire d'Alfort (ENVA) | This publication was funded by grants from The French Agency for Food, Environmental and Occupational Health and Safety (ANSES) (Cofunding Grant no. PHD2014-2017), the French Agricultural Research and International Cooperation Organization (CIRAD) (Cofunding Grant no. PHD2014-2017), and the French National Institute for Agricultural Research (INRAE) (Grant no. PATHO-ID Metaprogram MEM 2012-2014). This study was partly supported by the MALIN project “Surveillance, diagnosis, control and impact of infectious diseases of humans, animals and plants in tropical islands” supported by the European Union in the framework of the European Regional Development Fund (ERDF) and the Regional Council of Guadeloupe (Grant no. 2015-FED-186). The study used the ticks collected in Guadeloupe and Martinique within the framework of previous projects: PathoID “Rodent and tick Pathobiome” funded by INRAE and Resist project “Assessment of Tick Resistance to Acaricides in the Caribbean - Development of strategies to improve surveillance and control of tick-borne diseases in Ruminants” funded by the Fond de Cooperation Régionale (FCR) (Grant no. FCR2013/02) of Guadeloupe and Martinique. ST’s contribution was supported by the Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ (grant no. ANR-10-LABX-62-IBEID) and by the Direction Internationale de l’Institut Pasteur (Grant no. ST2019). | We thank the “Tiques et Maladies à Tiques (TMT)” group of the CNRS “Réseau Ecologie des Interactions Durables” for stimulating discussions and support. We thank J. Pradel, M. Hamon, S. Depraz, L. Felixine, R. Aprelon, J.F. Cosson and D. Pleydell for their contribution during tick collection. | ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
International audience
Mostrar más [+] Menos [-]Inglés. Ticks transmit a wide variety of pathogens including bacteria, parasites and viruses. Over the last decade, numerous novel viruses have been described in arthropods, including ticks, and their characterization has provided new insights into RNA virus diversity and evolution. However, little is known about their ability to infect vertebrates. As very few studies have described the diversity of viruses present in ticks from the Caribbean, we implemented an RNA-sequencing approach on Amblyomma variegatum and Rhipicephalus microplus ticks collected from cattle in Guadeloupe and Martinique. Among the viral communities infecting Caribbean ticks, we selected four viruses belonging to the Chuviridae, Phenuiviridae and Flaviviridae families for further characterization and designing antibody screening tests. While viral prevalence in individual tick samples revealed high infection rates, suggesting a high level of exposure of Caribbean cattle to these viruses, no seropositive animals were detected. These results suggest that the Chuviridae-and Phenuiviridae-related viruses identified in the present study are more likely tick endosymbionts, raising the question of the epidemiological significance of their occurrence in ticks, especially regarding their possible impact on tick biology and vector capacity. The characterization of these viruses might open the door to new ways of preventing and controlling tick-borne diseases.
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