CLONING AND EXPRESSION OF ENDOGLUCANASE GENE FROM TERMITE GUT BACTERIA FOR IMPROVING FIBRE DIGESTION *
2024
N.V. Aruna | K.Vijayarani
The present study describes the cloning and expression of endoglucanase gene from termite gut bacteria. Salmonella enterica with cellulolytic activity was isolated from the gut of the termite Odontotermes formosanus and characterized by sugar fermentation tests, biochemical tests and 16S rRNA sequencing. Endoglucanase gene was amplified from the genomic DNA of the bacteria and the purified 1.72 Kbp Polymerase chain reaction product was cloned into pET100 prokaryotic vector. The recombinant plasmid expressed a 65kDa endoglucanase which reacted with anti His-HRP antibody in western blotting. Cellulolytic activity of the recombinant plasmid was confirmed by congo red assay and the enzyme activity was found to be 54 Units/ml.
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