Development of Whole Genome Sequence-based Novel SSR Markers in Astragalus membranaceus (Fisch.)
2021
Hur, M. | Um, Y.R. | Lee, Y. | Lee, Y.J. | Koo, S.C. | Park, W.T. | Kim, J.H. | Huh, Y.C. | Moon, Y.H.
BACKGROUND: Although Astragalus membranaceus is a major medicinal herb used for food and medicinal purposes, genome-based research for its exploration is insufficient. The introduction of the genetic breeding system for medicinal crops, based on genomic analysis, will have a great impact not only on the establishment of the origin but also on understanding the existing traditional breeding system. METHODS AND RESULTS: A. membranaceus from five accessions of Poongsung, Asung, Jecheon, Sancheong, and Jilin from China were used, which are cultivated in the test field. The produced nucleotide sequences were assembled de novo to obtain 450,449 contigs, and the repeat sequences were extracted using the selection criteria, two to six nucleotide sequences repeated five time or more. Finally, we obtained 147,766 simple sequence repeat (SSR) marker candidates. Using the CLC genomics workbench program, 949 SSR markers showing mutations for Astragalus in the 5 accessions were selected. From the designed primer sets, 99 were randomly selected and applied to the genomic DNA which was extracted from five cultivars and pooled. Ten primer sets showing more than two bands were finally selected as SSR markers, and were used for the genetic relationship analysis in 81 strains of A. membranaceus. CONCLUSIONS: The data of this study can be used as a marker when registering A. membranaceus varieties through pure line selection and line breeding in the future. It is thought that it can greatly contribute to securing intellectual property rights for domestic Astragalus breeding varieties.
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