B cells response directed against Cut4 and CFP21 lipolytic enzymes in active and latent tuberculosis infections
2018
Rénier, Wendy | Bourdin, Arnaud | Rubbo, Pierre-Alain | Peries, Marianne | Dedieu, Luc | Bendriss, Sophie | Kremer, Laurent | Canaan, Stéphane | Terru, Dominique | Godreuil, Sylvain | Nagot, Nicolas | van de Perre, Philippe | Tuaillon, Edouard | Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier) | Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) | Aix Marseille Université (AMU) | Sécurité et Qualité des Produits d'Origine Végétale (SQPOV) ; Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU) | Pathogénèse et contrôle des infections chroniques (PCCI) ; Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM) | Dynamique des interactions membranaires normales et pathologiques (DIMNP) ; Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
International audience
Mostrar más [+] Menos [-]Inglés. BackgroundBetter understanding of the immune response directed against Mycobacterium tuberculosis (Mtb) is critical for development of vaccine strategies and diagnosis tests. Previous studies suggested that Mtb enzymes involved in lipid metabolism, are associated with persistence and/or reactivation of dormant bacilli.MethodsCirculating antibodies secreting cells (ASCs), memory B cells, and antibodies directed against Cut4 (Rv3452) and CFP21 (Rv1984c) antigens were explored in subjects with either active- or latent-tuberculosis (LTB), and in Mtb-uninfected individuals.ResultsCirculating anti-Cut4 ASCs were detected in 11/14 (78.6%) subjects from the active TB group vs. 4/17 (23.5%) from the LTB group (p = 0.001). Anti-CFP21 ASCs were found in 11/14 (78.6%) active TB vs. in 5/17 (29.4%) LTB cases (p = 0.01). Circulating anti-Cut4 and anti-CFP21 ASCs were not detected in 38 Mtb uninfected controls. Memory B cells directed against either Cut4 or CFP21 were identified in 8/11 (72.7%) and in 9/11 (81.8%) subjects with LTB infection, respectively, and in 2/6 Mtb uninfected individuals (33.3%). High level of anti-Cut4 and anti-CFP21 IgG were observed in active TB cases.ConclusionCirculating IgG SCs directed against Cut4 or CFP21 were mostly detected in patients presenting an active form of the disease, suggesting that TB reactivation triggers an immune response against these two antigens.
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