Conserved white rot enzymatic mechanism for wood decay in the Basidiomycota genus Pycnoporus
2020
Miyauchi, Shingo | Hage, Hayat | Drula, Elodie | Lesage-Meessen, Laurence | Berrin, Jean-Guy | Navarro, David | Favel, Anne | Chaduli, Delphine | Grisel, Sacha | Haon, Mireille | Piumi, François | Levasseur, Anthony | Lomascolo, Anne | Ahrendt, Steven | Barry, Kerrie | Labutti, Kurt | Chevret, Didier | Daum, Chris | Mariette, Jérôme, J. | Klopp, Christophe | Cullen, Daniel | de Vries, Ronald | Gathman, Allen | Hainaut, Matthieu | Henrissat, Bernard | Hildén, Kristiina | Kues, Ursula | Lilly, Walt | Lipzen, Anna | Mäkelä, Miia | Martinez, Angel | Morel-Rouhier, Melanie | Morin, Emmanuelle | Pangilinan, Jasmyn | Ram, Arthur | Wösten, Han | Ruiz-Dueñas, Francisco | Riley, Robert | Record, Eric | Grigoriev, Igor | Rosso, Marie-Noelle | Biodiversité et Biotechnologie Fongiques (BBF) ; Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Interactions Arbres-Microorganismes (IAM) ; Université de Lorraine (UL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité) | Microbes Evolution Phylogénie et Infections (MEPHI) ; Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU) | Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille) | U.S. Department of Energy [Washington] (DOE) | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRAE) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | USDA Forest Service Rocky Mountain Forest and Range Experiment Station ; United States Department of Agriculture (USDA) | Westerdijk Fungal Biodiversity Institute [Utrecht] (WI) ; Royal Netherlands Academy of Arts and Sciences (KNAW) | Helsingin yliopisto = Helsingfors universitet = University of Helsinki | Missouri Southern State University (MSSU) | Architecture et fonction des macromolécules biologiques (AFMB) ; Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Department of Molecular Cell Biology [Göttingen] ; Max Planck Institute for Biophysical Chemistry (MPI-BPC) ; Max-Planck-Gesellschaft-Max-Planck-Gesellschaft | Department of Energy / Joint Genome Institute (DOE) ; Los Alamos National Laboratory (LANL) | Centro de Investigaciones Biológicas (CSIC) ; Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] (CSIC) | DOE Joint Genome Institute [Walnut Creek] | Department of Molecular Microbiology and Biotechnology ; Tel Aviv University (TAU) | Universiteit Utrecht / Utrecht University [Utrecht] | Department of Plant Pathology & Microbiology [Riverside] ; University of California [Riverside] (UC Riverside) ; University of California (UC)-University of California (UC) | Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231Ministerio de Ciencias, Innovación y Universidades de España (BIO2017-86559-R) | ANR-14-CE06-0020,FUNTUNE,Cocktails enzymatiques inspirés de modèles fongiques pour la déconstruction contrôlée de la biomasse végétale(2014)
International audience
Mostrar más [+] Menos [-]Inglés. White-rot (WR) fungi are pivotal decomposers of dead organic matter in forest ecosystems and typically use a large array of hydrolytic and oxidative enzymes to deconstruct lignocellulose. However, the extent of lignin and cellulose degradation may vary between species and wood type. Here we combined comparative genomics, transcriptomics and secretome proteomics to identify conserved enzymatic signatures at the onset of wood decaying activity within the Basidiomycota genus Pycnoporus. We observed strong conservation in the genome structures and the repertoires of protein coding genes across the four Pycnoporus species described to date, despite the species having distinct geographic distributions. We further analyzed the early response of P. cinnabarinus, P. coccineus and P. sanguineus to diverse (ligno)-cellulosic substrates. We identified a conserved set of enzymes mobilized by the three species for breaking down cellulose, hemicellulose and pectin. The co-occurrence in the exo-proteomes of H2O2 producing enzymes with H2O2 consuming enzymes was a common feature of the three species, although each enzymatic partner displayed independent transcriptional regulation. Finally, cellobiose dehydrogenase-coding genes were systematically co-regulated with at least one AA9 LPMO gene, indicative of enzymatic synergy in vivo. This study highlights a conserved core white-rot fungal enzymatic mechanism behind the wood decaying process.
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