Oil assessment of Halamphora coffeaeformis diatom growing in a hybrid two-stage system for biodiesel production

The lipid content, composition and productivity of the marine benthic diatom Halamphora coffeaeformis were studied in order to evaluate its potential as feedstock for biodiesel production. Cultures were carried out in two stages: I) in photobioreactors (PBRs) to increase the biomass as inoculum for larger volumes, and II) in raceway ponds to increase naturally the triacylglycerol (TAG) content during the stationary growth phase. Biomass concentrations of 0.64 g L-1 and 0.23 g L-1 were reached in the PBR and the raceway pond, respectively. Total lipid content was 54.4 (+11.6) % ash free dry weight (AFDW) in the raceway pond on day 19 (harvest day), with a neutral lipid content of 34% AFDW. The TAG productivity in the raceway pond was 1.2 mg L-1 d-1. The indicators of biodiesel, calculated from fatty acid profile composition, showed that H. coffeaeformis oil was of good quality, according to international standards. Some hypothetical aspects are proposed in order to improve lipid productivity and net energy ratio in processes at larger scales.

Fil: Martin, Lucas Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina

Fil: Popovich, Cecilia Angelines. Provincia de Buenos Aires. Dirección Gral. de Cultura y Educación. Universidad Provincial del Sudoeste. Centro de Emprendedorismo y Desarrollo Territorial Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina

Fil: Martínez, Ana M.. Universidad Nacional del Sur. Departamento de Química; Argentina

Fil: Damiani, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina

Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina