Pathogenic potential of the surviving Salmonella Enteritidis on strawberries after disinfection treatments based on ultraviolet-C light and peracetic acid

Fresh fruits, especially strawberries, are usually consumed raw without any step to ensure their food safety. Salmonella enterica is one of the most important etiologic agents for foodborne diseases throughout the world and its ability to respond to some stress responses makes it even more dangerous. In the present investigation, we study the survival of S. Enteritidis (CECT-4300) on strawberries after 2-min of various disinfection steps (NaClO (200 ppm), peracetic acid (PAA; 40 ppm), water-assisted UV-C (WUV-C), and the combination WUV-C and 40 ppm of PAA (WUV-C + PAA)) and after 5 days of cold storage (4 °C). Moreover, the pathogenic potential of the surviving bacteria, such as the ability to survive throughout the gastrointestinal tract (GI) and subsequently the capability to adhere to and invade Caco-2 cells, was tested at each sampling point. After 2-min of washing procedures, reductions of S. Enteritidis on strawberries were ≥1.2 log, with no significant differences among treatments. However, the use of WUV-C + PAA treatment achieved the highest reductions in washing water, in which S. Enteritidis was not detected (<DL). At the end of 5-day storage period, reductions of the WUV-C + PAA treated-samples were lower in comparison with PAA-treated samples (P < 0.05). The effect of the treatments used did not interfere with the survival of S. Enteritidis along the GI tract. After disinfection and subsequent GI simulation, all remaining populations demonstrated an elevated ability to adhere in Caco-2 cells (67.5-81.1%) compared with those obtained on untreated strawberries (61.4%). Concerning the ability of invasion, no significant differences could be observed. Remarkably, after 5 days at 4 °C, the adherence of S. Entertidis significantly decreased in the samples with the combined treatment meanwhile the invasion ability was not detected for any treatment. The results of the present study are essential for the quantitative microbial risk estimations.

The authors are thankful to the Spanish Government ‘Ministerio de Economía y Competitividad’ (research project FRESAFE AGL2016-78086-R) and the CERCA Programme of ‘Generalitat de Catalunya’ for its financial support. J. Ortiz-Solà and I. Nicolau-Lapeña are grateful to the University of Lleida and the ‘Ministerio de Economía, Industria y Competitividad’ for their grant numbers BOU186 - 243/2017 UdL and BES-2017-079779, respectively.