OsJAR1 is required for JA-regulated floret opening and anther dehiscence in rice
2014
Xiao, Y. G. | Chen, Y. | Charnikhova, T. | Mulder, P. P. J. | Heijmans, J. | Hoogenboom, A. | Agalou, A. | Michel, Corinne | Morel, Jean-Benoit, J.-B. | Dreni, L. | Kater, M. M. | Bouwmeester, H. | Wang, M. | Zhu, Z. | Ouwerkerk, P. B. F. | Universiteit Leiden = Leiden University | Chinese Academy of Sciences (CAS) | Plant Research Institute BV ; Partenaires INRAE | Wageningen UR ; Alterra Green World Research (ALTERRA) | Biologie et Génétique des interactions Plantes-parasites pour la Protection Intégrée ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Università degli Studi di Milano = University of Milan (UNIMI) | Plant Research International ; Wageningen University and Research [Wageningen] (WUR) | The Netherlands Organisation for Applied Scientific Research (TNO) | Netherlands of the Royal Netherlands Academy of Arts and Sciences (KNAW) : 04-PSA-BD-04 ; China Exchange Programme : KNAW-CEP, 04CDP022, 06CDO033, 07CDP005, 08CDP042 ; Netherlands Organization for Scientific Research (NWO; VICI-grant)
BGPI : équipe 4
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Mostrar más [+] Menos [-]Inglés. Jasmonates are important phytohormones regulating reproductive development. We used two recessive rice Tos17 alleles of OsJAR1, osjar1-2 and osjar1-3, to study the biological function of jasmonates in rice anthesis. The florets of both osjar1 alleles stayed open during anthesis because the lodicules, which control flower opening in rice, were not withering on time. Furthermore, dehiscence of the anthers filled with viable pollen, was impaired, resulting in lower fertility. In situ hybridization and promoter GUS transgenic analysis confirmed OsJAR1 expression in these floral tissues. Flower opening induced by exogenous applied methyl jasmonate was impaired in osjar1 plants and was restored in a complementation experiment with transgenics expressing a wild type copy of OsJAR1 controlled by a rice actin promoter. Biochemical analysis showed that OsJAR1 encoded an enzyme conjugating jasmonic acid (JA) to at least Ile, Leu, Met, Phe, Trp and Val and both osjar1 alleles had substantial reduction in content of JA-Ile, JA-Leu and JA-Val in florets. We conclude that OsJAR1 is a JA-amino acid synthetase that is required for optimal flower opening and closing and anther dehiscence in rice.
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