Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis
2015
Gioffré, Andrea | Correa Muñoz, Magnolia | Alvarado Pinedo, María Fiorella | Vaca, Roberto José Antonio | Morsella, Claudia | Fiorentino, María Andrea | Paolicchi, Fernando | Ruybal, Paula | Zumárraga, Martín | Travería, Gabriel Eduardo | Romano, María Isabel
Multiple-locus variable number-tandem repeat analysis (MLVA) of <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS<i>900</i>-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also able to demonstrate the coexistence of genotypes in herds and co-infection at the organism level. This study shows that all the patterns described are common to those described in Europe, suggesting an epidemiological link between the continents.
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