Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in Arabidopsis and rice
2012
Evrard, A. | Bargmann, B. | Birnbaum, K. | Tester, M. | Baumann, U. | Johnson, A. | Shabala, S. | Cuin, T.
Fluorescence-activated cell sorting (FACS) provides a rapid means of isolating large numbers of fluorescently tagged cells from a heterogeneous mixture of cells. Collections of transgenic plants with cell type-specific expression of fluorescent marker genes such as green fluorescent protein (GFP) are ideally suited for FACS-assisted studies of individual cell types. Here we describe the use of Arabidopsis and rice enhancer trap lines with tissue-specific GFP expression patterns in the root to isolate specific cell types of root tissues using FACS. Additionally, protocols are provided to impose a ramped salinity stress for 48 h prior to cell sorting.
Mostrar más [+] Menos [-]Aurelie Evrard, Bastiaan O. R. Bargmann, Kenneth D. Birnbaum, Mark Tester, Ute Baumann, and Alexander A. T. Johnson
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