Seed dormancy in Camellia sinensis L. (Theaceae): Effects of cold-stratification and exogenous gibberellic acid application on germination
2016
Song, Danping | Jaganathan, Ganesh K. | Han, Yingying | Liu, Baolin
There are several different opinions about the presence of dormancy in tea (Camellia sinensis), but no strong evidence is available to conclude whether or not seeds are dormant. Freshly matured tea seeds collected from Hangzhou, China, at the natural dispersal time did not germinate in light at daily alternative temperature regimes of 10/15, 15/20, 20/25, 25/35ยบ C or at a constant temperature of 25ยบ C. Seeds were permeable to water and the embryos did not grow prior to radicle emergence, thus, the seeds have no physical, morphological or morphophysiological dormancy. When cold-stratified at 4ยบ C for 1, 2 and 3 months, 64, 88 and 93% of the seeds germinated respectively. Intact fresh seeds failed to germinate after treating with 0, 10, 500 and 1000 ppm GA3, whereas 3, 4, 61 and 86% of cracked seeds germinated respectively. Thus, the seeds have non-deep and intermediate physiological dormancy. Two-months cold-stratified seeds buried at 0, 1 and 5 cm soil depth in pots showed that seeds at 1 cm depth established significantly higher number of seedlings (P
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