In vitro solubilization of fat-soluble vitamins in structurally defined mixed intestinal assemblies
2021
El Aoud, Asma | Reboul, Emmanuelle | Dupont, Aurélien | Mériadec, Cristelle | Artzner, Franck | Marze, Sébastien | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN) ; Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Biosit : biologie, santé, innovation technologique (SFR UMS CNRS 3480 - INSERM 018) ; Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes (Biosit : Biologie - Santé - Innovation Technologique) | Institut de Physique de Rennes (IPR) ; Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS) | Agence Nationale de la Recherche | ANR-18-CE21-0002,AssemBiles,Structure d'assemblages mixtes lipides-sels biliaires et capacité de solubilisation/absorption de micro-constituants lipophiles(2018)
International audience
Mostrar más [+] Menos [-]Inglés. The structures of fed state intestinal assemblies containing bile components, dietary fat, and fat-solublevitamins are not well known, although they are involved in lipid transport. In this study, several methodswere used to investigate structural transitions upon various dietary lipids or various fat-soluble vitaminsincorporation in bile intestinal assemblies. In particular, DLS and turbidimetry were used to study tran-sition points as a function of component concentration, and cryo-TEM and SAXS were used to resolveassembly structures at microscopic and supramolecular scales, respectively. Results showed that increas-ing the concentration of dietary lipids in bile assembly induced a transition from core-shell micelles tounilamellar vesicles (except with caprylate lipids, always yielding micelles). In these specific assemblies,increasing the concentration of a fat-soluble vitamin either induced a systematic structural transition,defining a solubilization capacity (a-tocopherol or phylloquinone), or induced a structural transition onlyin micelles (retinol), or did not induce any structural transition up to very high concentrations (cholecal-ciferol). Using SAXS data, ideal molecular organizations are proposed for assemblies in the absence orpresence ofa-tocopherol.
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