The nitrate transporter family protein NPF8.6 controls the N-fixing nodule activity.
2017
Valkov, Vladimir Totev | Rogato, Alessandra | Alves, Ludovico Martins | Sol, Stefano | Noguero, Mélanie | Leran, Sophie | Lacombe, Benoît | Chiurazzi, Maurizio | Istituto di Bioscienze e BioRisorse = Institute of Biosciences and Bioresources (IBBR CNR) ; National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR) | Biochimie et Physiologie Moléculaire des Plantes (BPMP) ; Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | ANR-14-CE34-0007-01-HONIT | ANR-14-CE34-0007,HONIT,Hormone cross-talk drives nutrient dependent plant development(2014)
N-fixing nodules are new organs formed on legume roots as result of the beneficial interaction with soil bacteria, rhizobia. The nodule functioning is still a poorly characterized step of the symbiotic interaction as only few of the genes induced in N-fixing nodules have been functionally characterized. We present here the characterization of the member of the Lotus japonicus nitrate transporter 1/peptide transporter family LjNPF8.6. The phenotypic characterization carried out in independent L. japonicus LORE1 insertion lines indicates a positive role of LjNPF8.6 on nodule functioning as knock out mutants display N-fixation deficiency (25%) and increased nodular superoxide content. The partially compromised nodule functioning induces two striking phenotypes: anthocyanin accumulation already displayed four weeks after inoculation and shoot biomass deficiency, which is detected by long-term phenotyping. LjNPF8.6 achieves nitrate uptake in Xenopus laevis oocytes both at 0.5 mM and 30 mM external concentrations and a possible role as nitrate transporter on the control of N-fixing nodule activity is discussed.
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