Polymorphic microsatellite loci for the endangered pine hoverfly Blera fallax (Diptera: Syrphidae)
2012
Rotheray, Ellen | Greminger, Maja P | Nater, Alexander | Krutzen, Michael | Goulson, Dave | Bussiere, Luc | Biological and Environmental Sciences | University of Zurich | University of Zurich | University of Zurich | Biological and Environmental Sciences | Biological and Environmental Sciences | 0000-0001-8937-8381
We describe eleven polymorphic microsatellite loci developed for Blera fallax using ‘next generation’ 454 whole genome shotgun sequencing, along with conditions for three multiplex PCR reactions. We tested allelic variation on forty B. fallax individuals from Scotland and Sweden. Allelic richness and expected heterozygosity were 3.03 (±0.274) and 0.391 (±0.057) respectively. The number of alleles per locus ranged from 2 to 6. We anticipate that these loci will assist conservation management by allowing the monitoring of translocated populations, estimating effective population size, and assessing population structure and dispersal in Scotland and across Europe.
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