Photosynthetic parameters of Ulmus minor plantlets affected by irradiance during acclimatization
2013
Dias, M. C. | Pinto, G. | Correia, C. M. | Moutinho-Pereira, J. | Silva, S. | Santos, C.
In order to set up large-scale acclimatization protocols of micropropagated plants, an in-depth knowledge of their physiological responses during in vitro to ex vitro transfer is required. This work describes the photosynthetic performance of Ulmus minor micropropagated plants during acclimatization at high irradiance (HI; 200 ± 20 μmol m-2 s-1 or low irradiance (LI; 100 ± 20 μmol m-2 s-1). During this experiment, leaf pigment content, chlorophyll a fluorescence, gas exchange, stomata morphology, the activity of the Calvin cycle enzymes and saccharides were measured in persistent and new leaves. The results indicated that HI induces a higher photosynthetic performance compared to LI. Therefore, plants acclimatized under HI are likely to survive better after field transfer.
Mostrar más [+] Menos [-]This work was supported by the Portuguese Foundation for Science and Technology through a post-doctoral fellowship of M.C. Dias (SFRH/BPD/41700/2007) and S. Silva (SFRH/BPD/74299/2010). The authors acknowledge Dr. K. Bennett for her assistance in proofreading the manuscript.
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