Comparative Evaluation of the Foot-and-Mouth Disease Virus Permissive LF-BK α<sub>V</sub>β<sub>6</sub> Cell Line for Senecavirus A Research
2022
Jessica Mason | Victoria Primavera | Lauren Martignette | Benjamin Clark | Jose Barrera | Janine Simmons | William Hurtle | John G. Neilan | Michael Puckette
Senecavirus A (SVA) is a member of the family <i>Picornaviridae</i> and enzootic in domestic swine. SVA can induce vesicular lesions that are clinically indistinguishable from Foot-and-mouth disease, a major cause of global trade barriers and agricultural productivity losses worldwide. The LF-BK α<sub>V</sub>β<sub>6</sub> cell line is a porcine-derived cell line transformed to stably express an α<sub>V</sub>β<sub>6</sub> bovine integrin and primarily used for enhanced propagation of Foot-and-mouth disease virus (FMDV). Due to the high biosecurity requirements for working with FMDV, SVA has been considered as a surrogate virus to test and evaluate new technologies and countermeasures. Herein we conducted a series of comparative evaluation in vitro studies between SVA and FMDV using the LF-BK α<sub>V</sub>β<sub>6</sub> cell line. These include utilization of LF-BK α<sub>V</sub>β<sub>6</sub> cells for field virus isolation, production of high virus titers, and evaluating serological reactivity and virus susceptibility to porcine type I interferons. These four methodologies utilizing LF-BK α<sub>V</sub>β<sub>6</sub> cells were applicable to research with SVA and results support the current use of SVA as a surrogate for FMDV.
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