ISOLASI PROTEIN BIJI LAMTORO GUNG (Leucaena leucocephala) MENGGUNAKAN CAIRAN RUMEN DOMBA

<!-- P { margin-bottom: 0.08in; } --> <p style="text-indent: 0.5in; margin-bottom: 0in; line-height: 100%;"><span style="font-family: Arial,sans-serif;"><em><strong>Abstract</strong></em></span><span style="font-family: Arial,sans-serif;"><em><strong> </strong></em></span></p> <p style="text-indent: 0.5in; margin-bottom: 0in; line-height: 100%;"><span style="font-family: Arial,sans-serif;">Proteins</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">are needed</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">in the community</span><span style="font-family: Arial,sans-serif;">, </span><span style="font-family: Arial,sans-serif;">either</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">as</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">a direct</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">intake</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">in the body</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">and</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">is used</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">in food products</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">to</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">improve their quality</span><span style="font-family: Arial,sans-serif;">. </span><span style="font-family: Arial,sans-serif;">Proteins</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">in</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">food can be</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">isolated</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">using</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">several</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">methods</span><span style="font-family: Arial,sans-serif;">, one </span><span style="font-family: Arial,sans-serif;">can use the</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">method of</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">enzymatic</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">hydrolysis</span><span style="font-family: Arial,sans-serif;">. </span><span style="font-family: Arial,sans-serif;">The purpose of</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">this study was</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">to</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">determine the effect of</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">enzyme concentration</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">and</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">rumen fluid</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">incubation time of</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">the protein content</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">and</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">functional properties</span><span style="font-family: Arial,sans-serif;">. </span><span style="font-family: Arial,sans-serif;">This research was conducted</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">using a variable</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">2</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">factors:</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">The first factor</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">enzyme concentrations</span><span style="font-family: Arial,sans-serif;">: </span><span style="font-family: Arial,sans-serif;">0</span><span style="font-family: Arial,sans-serif;">%, </span><span style="font-family: Arial,sans-serif;">40</span><span style="font-family: Arial,sans-serif;">%, </span><span style="font-family: Arial,sans-serif;">80</span><span style="font-family: Arial,sans-serif;">% </span><span style="font-family: Arial,sans-serif;">and</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">100</span><span style="font-family: Arial,sans-serif;">%. </span><span style="font-family: Arial,sans-serif;">Factor</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">II</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">incubation time</span><span style="font-family: Arial,sans-serif;">: </span><span style="font-family: Arial,sans-serif;">24 hours</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">and</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">48</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">hours</span><span style="font-family: Arial,sans-serif;">. </span><span style="font-family: Arial,sans-serif;">The higest yield protein results</span><span style="font-family: Arial,sans-serif;"> was </span><span style="font-family: Arial,sans-serif;">obtained</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">Leucaena</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">gung</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">seed</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">protein</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">concentrates</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">on</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">the concentration of</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">enzyme</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">treatment of</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">rumen fluid</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">(</span><span style="font-family: Arial,sans-serif;">10ml </span><span style="font-family: Arial,sans-serif;">/</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">100g</span><span style="font-family: Arial,sans-serif;">) </span><span style="font-family: Arial,sans-serif;">and a</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">24-</span><span style="font-family: Arial,sans-serif;">hour </span><span style="font-family: Arial,sans-serif;">incubation</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">time</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">has</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">a dry weight</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">protein content</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">56.30</span><span style="font-family: Arial,sans-serif;">%, on </span><span style="font-family: Arial,sans-serif;">oil</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">absorption capacity</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">of 1.58</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">ml</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">/</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">g</span><span style="font-family: Arial,sans-serif;">, </span><span style="font-family: Arial,sans-serif;">the power</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">of froth</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">11</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">,</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">56</span><span style="font-family: Arial,sans-serif;">%, </span><span style="font-family: Arial,sans-serif;">while for the</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">water absorption</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">of 3.42</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">ml</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">/</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">g</span><span style="font-family: Arial,sans-serif;">, </span><span style="font-family: Arial,sans-serif;">a density</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">of 0.56</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">g</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">Kamba</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">/</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">ml</span><span style="font-family: Arial,sans-serif;">, </span><span style="font-family: Arial,sans-serif;">the power</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">emulsion</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">52.75</span><span style="font-family: Arial,sans-serif;">%, </span><span style="font-family: Arial,sans-serif;">water content</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">6.39%</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">best results</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">obtained</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">from the</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">treatment of</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">the sheep</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">rumen fluid</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">concentrations of</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">enzyme</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">(</span><span style="font-family: Arial,sans-serif;">10ml </span><span style="font-family: Arial,sans-serif;">/</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">100g</span><span style="font-family: Arial,sans-serif;">) </span><span style="font-family: Arial,sans-serif;">and</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">incubation</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">time</span><span style="font-family: Arial,sans-serif;"> </span><span style="font-family: Arial,sans-serif;">of 48 hours</span></p> <p style="text-indent: 0.06in; margin-bottom: 0in; line-height: 100%;">Keywords: Proteins, enzymes rumen, Lamtoro gung, incubation</p> <p style="text-indent: 0.44in; margin-bottom: 0in; line-height: 100%;"> </p> <p style="text-indent: 0.44in; margin-bottom: 0in; line-height: 100%;"><span style="font-family: Arial,sans-serif;"><strong>Abstract</strong></span></p> <p style="text-indent: 0.44in; margin-bottom: 0in; line-height: 100%;"><span style="font-family: Arial,sans-serif;">Protein banyak dibutuhkan dalam masyarakat, baik sebagai asupan langsung dalam tubuh maupun digunakan dalam produk pangan untuk meningkatkan kualitasnya. Protein dalam bahan pangan dapat diisolasi menggunakan beberapa metode, salah satunya dapat menggunakan metode hidrolisis enzimatis. Tujuan penelitian ini adalah untuk mengetahui pengaruh konsentrasi enzim cairan rumen domba dan lama inkubasi terhadap kadar protein dan sifat fungsional dari pekatan protein biji lamtoro gung. Penelitian ini dilakukan menggunakan variable 2 faktor, yaitu : Faktor I konsentrasi enzim : 0%, 40%, 80% dan 100%. Faktor II lama inkubasi: 24 jam dan 48 jam. Hasil penelitian terbaik didapatkan pekatan protein biji lamtoro gung tertinggi pada perlakuan konsentrasi enzim cairan rumen domba (10ml/100gr) dan lama inkubasi 24 jam memiliki kadar protein berat kering 56,30%, daya serap minyak 1,58 ml/gr, daya buih 11,56%, sedangkan untuk daya serap air 3,42 ml/gr, densitas kamba 0,56 gr/ml, daya emulsi 52,75%, kadar air 6,39% hasil terbaik didapatkan dari perlakuan konsentrasi enzim cairan rumen domba (10ml/100gr) dan lama inkubasi 48 jam.</span></p> <p style="margin-bottom: 0in; line-height: 100%;"><span style="font-family: Arial,sans-serif;">Kata Kunci: Protein, enzim rumen, Lamtoro gung, inkubasi </span></p>