<i>ty-5</i> Confers Broad-Spectrum Resistance to Geminiviruses
2022
Yanxiang Ren | Xiaorong Tao | Dawei Li | Xiuling Yang | Xueping Zhou
The selection of resistant crops is an effective method for controlling geminivirus diseases. <i>ty-5</i> encodes a messenger RNA surveillance factor Pelota with a single amino acid mutation (Pelota<sup>V16G</sup>), which confers effective resistance to tomato yellow leaf curl virus (TYLCV). No studies have investigated whether <i>ty-5</i> confers resistance to other geminiviruses. Here, we demonstrate that the tomato <i>ty-5</i> line exhibits effective resistance to various geminiviruses. It confers resistance to two representative begomoviruses, tomato yellow leaf curl China virus/tomato yellow leaf curl China betasatellite complex and tomato leaf curl Yunnan virus. The <i>ty-5</i> line also exhibits partial resistance to a curtovirus beet curly top virus. Importantly, <i>ty-5</i> confers resistance to TYLCV with a betasatellite. Southern blotting and quantitative polymerase chain reaction analyses showed that significantly less DNA of these geminiviruses accumulated in the <i>ty-5</i> line than in the susceptible line. Moreover, knockdown of <i>Pelota</i> expression converted a <i>Nicotiana benthamiana</i> plant from a geminivirus-susceptible host to a geminivirus-resistant host. Overall, our findings suggest that <i>ty-5</i> is an important resistance gene resource for crop breeding to control geminiviruses.
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