Crambe tataria sebeók seeds and plants grown in vitro and in vivo fatty acid composition comparison
2016
Nadia Oleksandrivna Pushkarova | Maria Sergiivna Kalista | Maxim Andriyovich Kharkhota | Dzhamal Rakhmetov | Mukola Viktorovich Kuchuk
<p class="PotText" style="line-height: 115%;">Methods of<em> in vitro</em> conservation offer a number of advantages for endangered species preservation. <em>Crambe tataria</em> Sebeók biochemistry study (fatty acid (FA) composition, antioxidant activity (AOA), polyfructan and total soluble protein content) is fairly importaint and could show the potential value of this species in agriculture, Food and Chemical Industry or pharmacology including its use as a source of valuble genetic material and could lead to new promising sources of biofuel discovery. Also, comparison of <em>in vitro</em> and <em>in vivo</em> cultured plants could point out to the effect of <em>in vitro</em> culture methods on plants biochemical composition Fatty acid (FA) content was determined using Gas<em> </em>chromatography-mass spectrophotometry (GC/MS) of fatty acid ethers. Antioxidant activity was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method. Total soluble protein content was measured using Bradford method and polyfructan content determination was based upon ketosugars ability to color in the acidic environment with resorcinol. Plants that were grown under <em>in vitro</em> and <em>in vivo</em> conditions and seeds were used in this research. Obtained data showed that <em>C. tataria</em> plants had high AOA and total soluble protein content along with high total FA content along with high content of α-Linolenic acid and absence of erucic acid. Difference in biochemical composition between plants grown in aseptic and not aseptic conditions was shown.</p><p> </p>
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